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KMID : 0363219960340020279
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Korean Journal of Dermatology
1996 Volume.34 No. 2 p.279 ~ p.288
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Effects of Helium-Neon Laser Irradiation on Proliferation and Collagen Synthesis by Human Dermal Fibroblasts Cultured in a Monolayer and Collagen Lattice
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서기범
윤소영/이우재/이증훈/박장규
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Abstract
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Background :
@EN Recently it has been suggested that lasers can modulate the biological functions of cells in vitro. It has also been reported that a helium-neon(He-Ne) laser can stimulate wound healing in the absence of thermal effects. However, the results
of
more
recent studies on the influence of He-Ne laser irradiation on the proliferation and collagen synthesis of human dermal fibroblastts have been inconclusive. And most experiments have not been performed in a three-dimensional collagen lattice that
is
the
physiologic model for an in vitro experiment.
@ES Objective :
@EN In this study we have investigated the natutre of influence of He-Ne laser irradiation on the proliferation and collagen synthesis of human dermal fibroblasts cultured in a monolayer and collagen lattice.
@ES Methods :
@EN We used a 10mW He-Ne laser emitting a beam of wavelength 632.8nm. The human dermal fibroblasts were subjected to laser treatment at various energy densities, and the treatment schedule included one daily exposure on three consecutive days.
DNA
replication was assessed by 3H-thymidine incorporation, and the collagen production was monitored by the synthesis of 3H-hydroxyproline following incubation of the culture with 3H-proline.
@ES Results :
@EN The results were as follows: In the control group, the proliferation and collagen synthesis of fibroblasts cultured in a collagen lattice on day 4 and 7 were significantly reduced compared with conventional monolayer cultures. Although the
proliferation of human dermal fibroblasts was not r3emarkable in all experimental irradiation energy except 4 J/cm2 in a monolayer culture, a statistically significant stimulating effect on fibroblasts proliferation in collagen lattice were noted
on
days 4 and 7 with an energy density of 1.5J/cm2. And we also found that a greater increase of collagen synthesis of human dermal fibroblasts occurred in a monolayer culture on days 4 and 7 in all irradiated energy densities than those in a
collagen
lattice culture. But statistically significant enhancement of collagen synthesis was showed only at the energy density of 1 J/cm2 in a collagen lattice culture on days 4.
@ES Conclusion :
@EN These data indicated that the influence of He-Ne laser irradiation on the proliferation and collagen synthesis of human dermal fibroblasts cultured in a collagen lattice was remarkably different from those in a monolayer culture In the
clinical
application of the He-Ne laser, the control of the amount of irradiation of the He-Ne laser may regulate the proliferative activity
(Kor J Dermatol 1996;34(1) 279~288)
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